CD4, clone: RPA-T4, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-0049-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The RPA-T4 monoclonal antibody reacts with human CD4, a 59kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: The RPA-T4 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. RPA-T4 has also been reported for use in blocking of CD4 activity in in vitro functional assays (Please use Functional Grade purified RPA-T4, cat. 16-0049, in functional assays.).Applications Tested: The RPA-T4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4|
|Flow CytometryþFunctional AssayþImmunohistochemistry|
|Store at 2-8°C.|
For Research Use Only.