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CD4, clone: RPA-T4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0049-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15206767

  • £60.53 / 100µg


Estimated Shipment
28-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The RPA-T4 monoclonal antibody reacts with human CD4, a 59kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). RPA-T4 blocks HIV binding and mixed lymphocyte reaction. The RPA-T4 antibody recognizes a different epitope than the OKT4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: The RPA-T4 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. RPA-T4 has also been reported for use in blocking of CD4 activity in in vitro functional assays (Please use Functional Grade purified RPA-T4, cat. 16-0049, in functional assays.).Applications Tested: The RPA-T4 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD4
RPA-T4
Unconjugated
Purified
Murine
100μg
Store at 2-8°C.
Monoclonal
Immunohistochemistry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Leu-3, T4
IgG1
RUO
Primary
Human
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