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CD4, Alexa Fluor 700, clone: OKT4 (OKT-4), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-0048-80

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15392100

  • £80.80 / 25µg


EU Stock 2
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Description

Description

Description: The OKT4 monoclonal antibody reacts with human CD4, a 59kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT4 (OKT-4) antibody is offered in 2 formats: -μg size: has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5μL (0.125μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.The Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD4
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P01730
CD4 antigen p55, CD4 receptor, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
OKT4 (OKT-4)
Alexa Fluor 700
CD4
Conjugated
CD4
IgG2b, kappa
25μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
920
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