missing translation for 'onlineSavingsMsg'
Learn More

CD3e, Biotin, clone: eBio500A2 (500A2), eBioscience™

Syrian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-0033-86

Code : Z2

Additional Details : Weight : 0.25000kg

 View more versions of this product

Product Code. 15299399

  • £281.05 / 1mg


Estimated Shipment date
from Supplier 03-04-2018
Add to basket

Disclaimers:

For Research Use Only.


Description

Description

Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and ε are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.Applications Reported: This eBio500A2 (500A2) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD3e
eBio500A2 (500A2)
Biotin
Conjugated
Golden Syrian Hamster
1mg
Store at 2-8°C. Do not freeze.
Monoclonal
Flow Cytometry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
CD3 epsilon
IgG
RUO
Primary
Murine
Videos