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CD3e, Biotin, clone: eBio500A2 (500A2), eBioscience™
Syrian Hamster Monoclonal Antibody
Brand: Affymetrix eBioscience 13-0033-86
Code : Z2
Additional Details : Weight : 0.25000kg
Description
Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and ε are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.Applications Reported: This eBio500A2 (500A2) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.Specifications
CD3e | |
Monoclonal | |
0.5mg/mL | |
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
P22646 | |
FLJ18683, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide TiT3 complex, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain | |
Golden Syrian Hamster | |
Affinity chromatography | |
RUO | |
Antibody | |
Monoclonal | |
Murine |
Flow Cytometry | |
eBio500A2 (500A2) | |
Biotin | |
Cd3e | |
Conjugated | |
Cd3e | |
IgG | |
1mg | |
Store at 2-8°C. Do not freeze. | |
Primary | |
12501 |
For Research Use Only.