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CD3e, Functional Grade, clone: 145-2C11, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0031-85

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15268337

  • £147.10 / 500µg


Estimated Shipment
29-05-2018
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Description

Description

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.Applications Reported: The 145-2C11 antibody has been reported for use in flow cytometric analysis. It has also been reported in cell activation and cell depletion. Please visit the Best Protocols webpage to view a protocol for in vitro T-cell activation.Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD3e
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P22646
FLJ18683, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide TiT3 complex, T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain
Armenian Hamster
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Functional Assay
145-2C11
Functional Grade
Cd3e
Purified
Cd3e
IgG
500μg
Store at 2-8°C.
Primary
12501
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