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CD3e, clone: 145-2C11, eBioscience™

Armenian Hamster Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0031-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15507416

  • £42.19 / 100µg


EU Stock 4
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For Research Use Only.


Description

Description

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.Applications Reported: The 145-2C11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported in cell activation and cell depletion. (Please use Functional Grade purified 145-2C11, cat. 16-0031, in functional assays.).Applications Tested: The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD3e
145-2C11
Unconjugated
Purified
Armenian Hamster
100μg
Store at 2-8°C.
Monoclonal
Western Blot
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
CD3 epsilon
IgG
RUO
Primary
Murine
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