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CD38, PerCP-eFluor™ 710, clone: 90, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-0381-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15331250

  • £206.18 / 100µg


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Description

Description

Description: The 90 monoclonal antibody reacts with the mouse CD38 molecule, an ∽42kDa type II transmembrane protein. CD38 is expressed at increasingly higher levels on B cells at each stage of B-cell differentiation, and is then down-regulated on germinal center B cells and mature plasma cells. Its expression is reported on a subpopulation of thymocytes, mature T cells, and NK cells. Crosslinking of CD38 on the surface of mature, resting B cells induces B-cell proliferation, which is enhanced by co-signals such as IL-4 and LPS. CD38, a counter-receptor for CD31, is an ectoenzyme with cyclase and hydrolase enzymatic activity and is speculated to play a role in lymphocyte activation and differentiation.Applications Reported: This 90 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 90 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD38
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P56528
T10, ADP-ribosyl cyclase 1, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase, CD38 antigen p45, NAD+ nucleosidase, cADPr hydrolase 1, cyclic ADP-ribose hydrolase 2
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
90
PerCP-eFluor 710
Cd38
Conjugated
Cd38
IgG2a, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12494
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