CD369 (Clec7a, Dectin-1), PE, clone: bg1fpj, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 12-5859-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The monoclonal antibody bg1fpj recognizes mouse CD369, also known as Dectin-1 or CLEC7A, a type II transmembrane protein in the C-type lectin family. The ligand is beta1, 3-glycan. Beta glycans are a main component in polysaccharides and specifically beta1,3-linked carbohydrates are found in the cell wall of fungi, plants and some bacteria. CD369 has been found to bind Candida, Aspergillis, Pneumocystis, Coccidioides and Mycobacterium. Calcium dependent binding to ligand signals through syk via the ITAM domain in the cytoplasmic portion and results in endocyotosis/phagocytosis and production of cytokines (IL-2, IL-6, IL-10, TNF alpha, IL-1 beta, IL-23). A direct role in Th17 and Th1 differentiation has been attributed to CD369 signaling. CD369 is expressed predominantly on myeloid cells (neutrophils, monocytes, macrophages, and dendritic cells). Low levels have also been reported on lymphocytes and mucosal epithelium.Applications Reported: This bg1fpj antibody has been reported for use in flow cytometric analysis.Applications Tested: This bg1fpj antibody has been tested by flow cytometric staining of mouse lysed whole blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|CD369 (Clec7A, Dectin-1)|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Beta-glucan receptor, ClecSF12|
For Research Use Only.