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CD369 (Clec7a, Dectin-1), PerCP-eFluor™ 710, clone: 15E2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-9856-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15549446

  • £91.01 / 25 tests


Estimated Shipment date
from Supplier 29-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: This 15E2 monoclonal antibody reacts with human and non-human primate CD369, which is also known as the Dectin-1 or beta-glucan receptor or CLEC7A. CD369 is expressed predominantly on monocytes, macrophages, and dendritic cells. Moreover, this receptor can also be detected on neutrophils and lymphocytes, as well as induced by cytokines such as IL-4 or LPS. In humans, CD369 exists as two isoforms that arise due to alternative splicing. CD369 binds beta-1,3-glucan and plays an important role in activating signal transduction involved in the innate immune response to fungal and mycobacterial infections. Studies have also shown the involvement of CD369 in dendritic cell activation.The 15E2 monoclonal antibody has been reported to recognize both isoforms of CD369 and activate dendritic cells. It does not crossreact to mouse CD369.Applications Reported: This 15E2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 15E2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5μL (0.25μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL cell sample + 100μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD369 (Clec7A, Dectin-1)
1.50E+03
PerCP-eFluor 710
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (0.25μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Beta-glucan receptor, ClecSF12
IgG2a
RUO
Primary
Non-human Primate
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