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CD369 (Clec7a, Dectin-1), Functional Grade, clone: 15E2, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-9856-85

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15537836

  • £199.70 / 500µg


Estimated Shipment date
from Supplier 31-05-2018
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Description

Description

Description: This 15E2 monoclonal antibody reacts with human and non-human primate CD369, which is also known as the Dectin-1 or beta-glucan receptor or CLEC7A. CD369 is expressed predominantly on monocytes, macrophages, and dendritic cells. Moreover, this receptor can also be detected on neutrophils and lymphocytes, as well as induced by cytokines such as IL-4 or LPS. In humans, CD369 exists as two isoforms that arise due to alternative splicing. CD369 binds beta-1,3-glucan and plays an important role in activating signal transduction involved in the innate immune response to fungal and mycobacterial infections. Studies have also shown the involvement of CD369 in dendritic cell activation.The 15E2 monoclonal antibody has been reported to recognize both isoforms of CD369 and activate dendritic cells. It does not crossreact to mouse CD369.Applications Reported: The 15E2 antibody has been reported to activate dendritic cells.Applications Tested: This 15E2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD369 (Clec7A, Dectin-1)
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
Q9BXN2
UNQ539/PRO1082, BGR, CANDF4, CLECSF12, DECTIN1,C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 12, C-type lectin domain family 7 member A, C-type lectin superfamily member 12, DC-associated C-type lectin 1, beta-glucan receptor, dectin-1, dendritic cell-associated C-type lectin 1, dendritic cell-associated C-type lectin-1, lectin-like receptor 1
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Non-human Primate
Functional Assay
1.50E+03
Functional Grade
CLEC7A
Purified
CLEC7A
IgG2a, kappa
500μg
Store at 2-8°C.
Primary
64581
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