CD366 (TIM3) Rat anti-Mouse, PE, Clone: 8B.2C12, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 12-5871-82

100UG ANTI-MOUSE TIM3 PE

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15288859

Quantity Price
1 £ 176.0 / 100µg
Estimated Shipment date
from Supplier 09-12-2016
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Description and Specification

Specification

Antigen CD366 (TIM3)
Applications Flow Cytometry
Clone 8B.2C12
Concentration 0.2mg/mL
Conjugate PE
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias HAVCR2, T cell immunoglobulin domain, mucin-like domain
Host Species Rat
Isotype IgG1
Quantity 100μg
Regulatory Status RUO
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

The 8B.2C12 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein. CD366 is a type I transmembrane protein and contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. CD366 is expressed selectively by differentiated CD4+ Th1 and CD8+ Tc1, but is absent on Th2 and Tc2. Other hematopoietic cell types, including naïve T cells, B cells, macrophages and dendritic cells, do not express CD366, at least at the protein level. Expression of CD366 is upregulated at a late stage of T cell differentiation on Th1 cells after 3 rounds of in vitro polarization suggesting a role for this molecule in the transport or effector function of Th1 cells rather than a contribution to T cell differentiation. In an experimental autoimmune encephalomyelitis (EAE) model, CD366 was shown to be expressed on most CD4+ and CD8+ T cells in the central nervous system at the onset of clinical signs of disease, while less than 2% of CD4+ cells in the periphery expressed CD366 after immunization. In this model, in vivo administration of 8B.2C12 resulted in a hyperacute and atypical disease phenotype. It is postulated that the engagement of CD366 during T cell activation results in the expansion and activation of macrophages and increased severity of autoimmune disease. The Tim gene family may have an important role in the regulation of autoimmunity and allergies.

The 8B.2C12 antibody binds to the BALB/c allele of CD366 while reactivity to the C57Bl/6 allele is significantly weaker.