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CD366 (TIM3), clone: RMT3-23, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-5870-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15277527

  • £62.63 / 50µg


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Description

Description

Description: The RMT3-23 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein. The RMT3-23 antibody reacts with CD366 protein expressed by both BALB/c and C57BL/6 strains of mice. CD366, a type I transmembrane protein, contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. CD366 is expressed selectively by differentiated CD4^+Th1 and CD8^+Tc1 cells, but is absent on CD4^+Th2 and CD8^+Tc2 cells. Other hematopoietic cell types, including naive T cells, B cells, macrophages and dendritic cells, do not express CD366, at least at the protein level. CD366 expression is upregulated at a late stage of T cell differentiation on Th1 cells after 3 rounds of in vitro polarization suggesting a role for this molecule in the transport or effector function of Th1 cells rather than a contribution to T cell differentiation. In an experimental autoimmune encephalomyelitis (EAE) model, CD366 was shown to be expressed on most CD4^+ and CD8^+ T cells in the central nervous system at the onset of clinical signs of disease, while less than 2% of CD4^+ cells in the periphery expressed CD366 after immunization.RMT3-23 has been shown to have functional activity; blocks DC recognition of apoptotic cells and also induces autoantibody production.Applications Reported: This RMT3-23 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RMT3-23 antibody has been tested by flow cytometric analysis of CD366 transfected cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD366
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
Q8VIM0
HAVCR2,TIM-3
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
RMT3-23
Unconjugated
Havcr2
Purified
Havcr2
IgG2a, kappa
50μg
Store at 2-8°C.
Primary
171285
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