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CD366 (TIM3), eFluor 450, clone: F38-2E2, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 48-3109-41
Code : NEW
Additional Details : Weight : 0.23750kg
Description
HAVCR2 is a 281 A.A Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. It consists of an IgV-like domain and a mucin-like domain in the extracellular region and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. HAVCR2 plays an important role in the regulation of macrophage activation and induction of autoimmune diseases. It down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of HAVCR2 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease.
Description: This F38-2E2 monoclonal antibody reacts with human CD366, also known as T cell immunoglobulin and mucin domain-containing protein 3 (TIM3) or HAVCR2. This cell surface receptor is expressed on activated CD4+ T cell subsets (e.g. Th1, Th17, and Treg), CD8+ T cells, monocytes, dendritic cells, and mast cells. Due to alternative splicing, CD366 exists as membrane-bound and soluble forms. Galectin-9 has been identified as the ligand for CD366. In humans, this receptor negatively regulates CD4+ T cells, influencing the secretion of some Th1- and Th17-related cytokines. CD366 has also been implicated in tolerance, autoimmune disease (e.g. multiple sclerosis), and HIV infection.Applications Reported: This F38-2E2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This F38-2E2 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5μL (0.5μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.Specifications
CD366 (TIM3) | |
Monoclonal | |
5μl/Test | |
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
Q8TDQ0 | |
HAVCR2 | |
Affinity chromatography | |
RUO | |
84868 | |
Store at 2-8°C. Do not freeze. Light-sensitive material. | |
Liquid |
Flow Cytometry | |
F38-2E2 | |
eFluor 450 | |
HAVCR2 | |
HAVCR2,TIM-3 | |
Mouse | |
25 Tests | |
Primary | |
Human | |
Antibody | |
IgG1 κ |
For Research Use Only.