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CD357 (AITR/GITR), PerCP-eFluor™ 710, clone: DTA-1, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 46-5874-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15599286

  • £209.03 / 100µg


Estimated Shipment date
from Supplier 28-06-2018
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Description

Description

Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.Applications Reported: This DTA-1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.004μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100μL cell sample + 100μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD357 (GITR)
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
O35714
AITR, GITR-D, TNFRSF18
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
DTA-1
PerCP-eFluor 710
Tnfrsf18
Conjugated
Tnfrsf18
IgG2b, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
21936
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