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CD357 (AITR/GITR), Functional Grade, clone: DTA-1, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-5874-83

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15238667

  • £239.32 / 200µg


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Description

Description

Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predominantly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease.Applications Reported: This DTA-1 antibody has been reported for use in flow cytometric analysis and immunohistochemistry of frozen mouse tissue. It has also been reported for use in functional assays.Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.03 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This DTA-1 antibody has been tested by immunohistochemistry of frozen tissue and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD357 (GITR)
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
O35714
AITR, GITR-D, TNFRSF18
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Functional Assay
DTA-1
Functional Grade
Tnfrsf18
Purified
Tnfrsf18
IgG2b, kappa
200μg
Store at 2-8°C.
Primary
21936
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