CD354 (TREM-1), eFluor 660, clone: TR3MBL1, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 50-3541-80
Code : NEW
Additional Details : Weight : 0.09500kg
DescriptionDescription: The TR3MBL1 monoclonal antibody recognizes mouse CD354 (also known as TREM-1), a 30-kDa glycosylated member of the Ig superfamily. CD354 is expressed on the cell surface of myeloid cells such as neutrophils, monocytes, dendritic cells, some macrophages, platelets, and megakaryocytes. Outside the immune system, CD354 is expressed on hepatocytes, osteoclasts, and microglia. CD354 also exists as a soluble receptor (sTREM-1) that arises from proteolytic cleavage of the membrane-bound receptor by matrix metalloproteases. CD354 plays a critical role in the innate immune response to bacterial and fungal infection by triggering the release of pro-inflammatory chemokines and cytokines. The cell surface receptor associates constitutively with the ITAM-containing adaptor protein DAP12 to activate the MAPK and NFkB signaling pathways. Finally, levels of the soluble receptor are increased in several inflammatory diseases, including sepsis, asthma, and inflammatory bowel disease, making this receptor a valuable diagnostic biomarker.Applications Reported: This TR3MBL1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This TR3MBL1 antibody has been tested by flow cytometric analysis of mouse peripheral blood cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|