CD35 Monoclonal Antibody (E11), FITC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-0359-42

Product Code. 15556386

Description

Description: This E11 monoclonal antibody reacts with human and non-human primate CD35, which is also known as complement receptor 1 (CR1). CD35 is expressed on B cells, T cell subsets, follicular dendritic cells, macrophages, neutrophils, eosinophils, and erythrocytes. This cell surface protein is also present on non-immune cells such as glomerular podocytes and Langerhans cells. CD35 binds the complement components C3b and C4b, which are derived from cleavage of C3 and C4, respectively, in the classical and alternative complement pathways. In humans, CD35 has been implicated in a variety of roles, including complement activation, transporting C3 complexes on erythrocytes, phagocytosis of complement-bound neutrophils and macrophages, B cell proliferation, and opsonization. Reports also indicate that Plasmodium falciparum, the pathogen that causes malaria, invades host erythrocytes by binding CD35. Decreased expression of CD35 on erythrocytes has been associated with systemic lupus erythematosus (SLE), type I diabetes, and rheumatoid arthritis. The E11 antibody has been reported to crossreact to capuchin monkey, chimpanzee, cynomologus monkey, olive baboon, and rhesus macaque. Applications Reported: This E11 antibody has been reported for use in flow cytometric analysis. Applications Tested: This E11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.06 μg) per test.

CD35 (CR1, Complement Receptor 1) is a cell membrane-bound, monomeric glycoprotein and its primary function is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. CD35 protein mediates cellular binding to particles and immune complexes that have activated complement. The CD35 complex is four different allotypes (C,A,B,D) C is 160 kDa; A is 190 kDa; B is 220 kDa and D is 250 kDa. CD35 is involved in the processing of immune complexes, promoting of binding and phagocytosis of C3b complexes and inhibition of complement activation. CD35 is on neutrophils, eosinophils, monocytes, B-cells, some NK-cells, erythrocytes, myeloid leukemias, follicular dendritic reticulum cells and is negative on basophils. Decreases in expression of CD35 protein and/or mutations in its gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus and sarcoidosis. Mutations in the CD35 gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. Alternate allele-specific splice variants encoding different isoforms of CD35 have been characterized. An additional secreted form of CD35 have also been described but has not been fully characterized.

Specifications

• Antigen: CD35
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P17927
• Gene Symbols: CR1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 1378
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: E11
• Conjugate: FITC
• Gene: CR1
• Gene Alias: C3 binding protein; C3b/C4b receptor; C3-binding protein; C3BR; C4BR; CD35; CD35 antigen; complement component (3b/4b) receptor 1 (Knops blood group); Complement Component (3b/4b) receptor 1 including Knops blood group system; complement component 3b/4b receptor 1 (Knops blood group); complement receptor type 1; CR1; KN; Knops blood group antigen
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human, Non-human Primate
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.