CD34 Rat anti-Mouse, eFluor 450, Clone: RAM34, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 48-0341-80

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15351710

Quantity Price
1 £ 74.51 / 25µg
EU Stock 12
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Description and Specification

Specification

Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Quantity 25μg
Clone RAM34
Antigen CD34
Host Species Rat
Isotype IgG2a
Monoclonal or Polyclonal Monoclonal
Conjugate eFluor 450
Format Conjugated
Applications Flow Cytometry
Regulatory Status RUO
Concentration 0.2mg/mL
Species Reactivity Mouse
Primary or Secondary Primary

The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin. It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells. CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity. CD34 expressed on endothelial cells is a ligand for CD62L and plays a role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers.