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CD34, clone: RAM34, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0341-81

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15296907

  • £42.19 / 50µg


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Disclaimers:

For Research Use Only.


Description

Description

Description: The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin. It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells. CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity. CD34 expressed on endothelial cells is a ligand for CD62L and plays a role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers.Applications Reported: This RAM34 antibody has been reported for use in flow cytometric analysis, immunohistology staining of frozen tissue sections (acetone-fixed only, not formalin-fixed), and immunohistology staining of paraffin embedded tissue sections.Applications Tested: This RAM34 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. (Fluorochrome-conjugated RAM34 is recommended for use in flow cytometry).Note: When staining with the RAM34 antibody an incubation time of 90 minutes is recommended to obtain the optimal signal to noise ratio.For more detailed information on staining with RAM34, please refer to Ema et al., listed under References.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD34
RAM34
Unconjugated
Purified
IgG2a
RUO
Primary
Murine
Immunohistochemistry (Frozen)
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Rat
50μg
Store at 2-8°C.
Monoclonal
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