CD34 Rat anti-Mouse, Clone: RAM34, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-0341-81

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only.

Product Code. 15296907

Quantity Price
1 £ 42.19 / 50µg
EU Stock 1
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Description and Specification

Specification

Isotype IgG2a
Quantity 50μg
Applications Flow Cytometry
Applications Immunohistochemistry (Formalin/Paraffin)
Applications Immunohistochemistry (Frozen)
Storage Requirements Store at 2-8°C.
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Species Reactivity Mouse
Host Species Rat
Format Purified
Concentration 0.5mg/mL
Regulatory Status RUO
Monoclonal or Polyclonal Monoclonal
Antigen CD34
Primary or Secondary Primary
Clone RAM34
Conjugate Unlabeled

The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin. It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells. CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity. CD34 expressed on endothelial cells is a ligand for CD62L and plays a role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers.