CD34 Rat anti-Mouse, Clone: RAM34, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 14-0341-82

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.01000kg



Disclaimers: For Research Use Only.

Product Code. 15206917

Quantity Price
1 £ 66.65 / 100µg
EU Stock 10
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Description and Specification

Specification

Applications Flow Cytometry
Applications Immunohistochemistry (Formalin/Paraffin)
Applications Immunohistochemistry (Frozen)
Antigen CD34
Isotype IgG2a
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Format Purified
Monoclonal or Polyclonal Monoclonal
Clone RAM34
Host Species Rat
Primary or Secondary Primary
Concentration 0.5mg/mL
Quantity 100μg
Species Reactivity Mouse
Regulatory Status RUO
Conjugate Unlabeled
Storage Requirements Store at 2-8°C.

The RAM34 monoclonal antibody reacts with mouse CD34, also known as mucosialin. It has been reported that the RAM34 antibody can be used to detect CD34+Sca-1+c-Kit+ cells. CD34 is a highly glycosylated (approximately 90-120 kDa) member of the sialomucin family and is expressed by capillary endothelial cells, bone marrow stroma, and a small subpopulation of mouse bone marrow cells. RAM34 has been used to purify mouse hematopoietic stem cells (HSC) to near homogeneity. CD34 expressed on endothelial cells is a ligand for CD62L and plays a role in adhesion. Simultaneous staining of mouse bone marrow cells with a cocktail of antibodies to lineage markers (CD3, CD11b, Ly6G, TER-119 and CD45R/B220) reveals a subset of cells that stain with the RAM34 antibody and express undetectable to low levels of the indicated lineage markers.