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CD34, FITC, clone: 4H11, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-0349-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15536386

  • £125.91 / 100 tests


EU Stock 4
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Description

Description

Description: The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of this family are single pass transmembrane proteins with a heavily glycosylated extracellular and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage by neuraminidase, chymopapain, or O-glycoprotease. According to this analysis, the 4H11 antibody belongs to class III, indicating that it reacts with a protein epitope.Applications Reported: This 4H11 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4H11 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD34
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P28906
Mucosialin, RP11-328D5.2, CD34 antigen, hematopoietic progenitor cell antigen CD34
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
4H11
FITC
CD34
Conjugated
CD34
IgG1, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
947
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