CD34, PE, clone: 1H6, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-0340-42
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The 1H6 monoclonal antibody reacts with canine CD34, also known as mucosialin. CD34 belongs to a protein family that also includes endoglycan and podocalyxin. These single-pass transmembrane proteins possess heavily glycosylated extracellular and N-terminal mucin domains. Similar to humans, canine CD34 can be detected on hematopoietic progenitor cells in the bone marrow, peripheral blood and hair follicle, as well as on some populations of mesenchymal stem cells, tumor cell lines, and on vascular endothelia in the adult. CD34 can be used as a marker to isolate cells capable of hematopoietic cell engraftment.The 1H6 antibody has been reported to work for enriching CD34+ cells.Applications Reported: This 1H6 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1H6 antibody has been pre-titrated and tested by flow cytometric analysis of a canine CD34-expressing cell line. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|