CD338 (ABCG2), PE, clone: 5D3, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-8888-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The 5D3 monoclonal antibody reacts with the extracellular portion of the human ABCG2 protein, also known as Bcrp1 and MXR. The ABCG2 gene, a member of the multi-drug resistance (MDR) family, is highly expressed on primitive 'side-population' (SP) stem cells, which are defined by the efflux of fluorescent dyes such as Rhodamine 123 and Hoechest 33342. In the bone marrow, about 0.05% of cells display the low fluorescence and are highly enriched for repopulating cells. The SP cells, which express low or undetectable levels of CD34, have been identified in multiple species. In addition, expression of ABCG2 appears to be highly conserved.Applications Reported: The 5D3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 5D3 antibody is tested by flow cytometric analysis of ABCG2 transfected cells and peripheral blood cells. It is offered in 2 formats: - μg size: has been tested by flow cytometric analysis of ABCG2 transfectant cells and peripheral blood. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of ABCG2 transfected cells and peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|ABCP, BCRP, BCRP1, MXR|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.