CD33, PE-Cyanine7, clone: 9A11-CD33, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-0331-82

Product Code. 15548386

Description

Description: This monoclonal antibody 9A11-CD33 reacts with mouse CD33, also known as Siglec3 and gp67. CD33 belongs to the sialic acid-binding immunoglobulin lectins (Siglec) family that binds to sialic acid-containing glycans on cell surface glycoproteins and glycolipids on mammalian cells. Mouse CD33 binds to short O-linked glycans of certain mucins and sialyl-Tn epitopes. Mouse CD33 is expressed by mature granulocytes, immature myeloid cells, and microglia. Unlike human CD33, mouse CD33 lacks a cytoplasmic ITIM domain and exists as two splice variants that have different cytoplasmic tails. In microglia, CD33 inhibits the phagocytosis of toxic amyloid beta.Applications Reported: This 9A11-CD33 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 9A11-CD33 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD33
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q63994
• Gene Alias: FLJ00391, SIGLEC-3, p67
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow Cytometry
• Clone: 9A11-CD33
• Conjugate: PE-Cyanine7
• Gene: Cd33
• Format: Conjugated
• Gene Symbols: Cd33
• Isotype: IgG1, kappa
• Quantity: 100μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures.
• Primary or Secondary: Primary
• Gene ID (Entrez): 12489

For Research Use Only.