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CD33, PE, clone: 9A11-CD33, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 12-0331-82

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15576756

  • £180.65 / 100µg


Estimated Shipment date
from Supplier 29-05-2018
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Description

Description

Description: This monoclonal antibody 9A11-CD33 reacts with mouse CD33, also known as Siglec3 and gp67. CD33 belongs to the sialic acid-binding immunoglobulin lectins (Siglec) family that binds to sialic acid-containing glycans on cell surface glycoproteins and glycolipids on mammalian cells. Mouse CD33 binds to short O-linked glycans of certain mucins and sialyl-Tn epitopes. Mouse CD33 is expressed by mature granulocytes, immature myeloid cells, and microglia. Unlike human CD33, mouse CD33 lacks a cytoplasmic ITIM domain and exists as two splice variants that have different cytoplasmic tails. In microglia, CD33 inhibits the phagocytosis of toxic amyloid beta.Applications Reported: This 9A11-CD33 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 9A11-CD33 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD33
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
Q63994
FLJ00391, SIGLEC-3, p67
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
9A11-CD33
PE
Cd33
Conjugated
Cd33
IgG1, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12489
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