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CD33, PE-Cyanine5, clone: HIM3-4, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 15-0339-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15557726

  • £77.09 / 25 tests


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Disclaimers:

For Research Use Only.


Description

Description

Description: The HIM3-4 monoclonal antibody reacts with human CD33, a 67kDa member of the sialoadhesion family. CD33 is expressed by myelomonocytic precursors, monocytes, mast cells, and granulocytes. Hematopoietic stem cells and lymphocytes do not express this antigen. CD33 plays a role in sialic-acid dependent cell adhesion.Applications Reported: The HIM3-4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIM3-4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD33
HIM3-4
PE-Cyanine5
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (1μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Sialic Acid-Binding Immunoglobulin-Like Lectin 3, SIGLEC3
IgG1
RUO
Primary
Human
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