CD325 (N-Cadherin), clone: 8C11, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-3259-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The 8C11 monoclonal antibody reacts with human CD325, also known as N-Cadherin. CD325 is a 130kDa member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently dysregulated in cancer cells.Applications Reported: This 8C11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.Applications Tested: This 8C11 antibody has been tested by flow cytometric analysis of HeLa cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The 8C11 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using low pH antigen retrieval at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Cadherin-2, Neural-cadherin, CDw325 antigen, CD325, CDHN, NCAD, Cadherin-N|
|Flow CytometryþImmunohistochemistryþImmunoprecipitationþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.