CD321 (F11R), clone: WK9, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-9321-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors. CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail. The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins. CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, epithelial and endothelial cells, and plays a role in mediating cell-cell contact. CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin. CD321 is also involved in reovirus entry into cells.Applications Reported: This WK9 antibody has been reported for use in flow cytometric analysis and immunoprecipitation. The WK9 antibody has also been found useful for immunoblotting, recognizing a protein of approximately 38 kD under reducing and non-reducing conditions.Applications Tested: This WK9 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|Human Junctional Adhesion Molecule-1 (JAM-1)|
|Flow CytometryþImmunoprecipitationþWestern Blot|
|Store at 2-8°C.|
For Research Use Only.