CD317 (BST2/PDCA-1) Rat anti-Mouse, PE-Cy7, Clone: eBio927, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 25-3172-80

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15538536

Quantity Price
1 £ 78.88 / 25µg
Estimated Shipment date
from Supplier 03-04-2017
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Description and Specification

Specification

Gene Alias plasmacytoid DC Ag-1
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Concentration 0.2mg/mL
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage
Isotype IgG2b
Primary or Secondary Primary
Clone eBio927
Format Conjugated
Monoclonal or Polyclonal Monoclonal
Quantity 25μg
Applications Flow Cytometry
Species Reactivity Mouse
Conjugate PE-Cy7
Host Species Rat
Antigen CD317 (BST2/PDCA-1)
Regulatory Status RUO

The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naïve mouse which represents a very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by antibody 120G8.

The eBio927 monoclonal antibody has also been shown to have functional activity. The epitope recognized by eBio927 is distinct from eBio129c; thus, the antibodies can be used to costain, purify and identify pDCs.