CD317 (BST2/PDCA-1) Rat anti-Mouse, FITC, Clone: eBio927, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 11-3172-81

UNSPSC: 12352200

Code: Z2

Additional Details:
Additional Details: Weight: 0.25000kg



Disclaimers: For Research Use Only.

Product Code. 15258199

Quantity Price
1 £ 54.42 / 50µg
Estimated Shipment date
from Supplier 04-05-2017
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Description and Specification

Specification

Primary or Secondary Primary
Species Reactivity Mouse
Quantity 50μg
Regulatory Status RUO
Concentration 0.5mg/mL
Clone eBio927
Isotype IgG2b
Format Conjugated
Applications Flow Cytometry
Conjugate FITC
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Antigen CD317 (BST2/PDCA-1)
Host Species Rat
Monoclonal or Polyclonal Monoclonal
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias plasmacytoid DC Ag-1

The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naïve mouse which represents a very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by antibody 120G8.

The eBio927 monoclonal antibody has also been shown to have functional activity. The epitope recognized by eBio927 is distinct from eBio129c; thus, the antibodies can be used to costain, purify and identify pDCs.