CD317 (BST2/PDCA-1) Rat anti-Mouse, APC, Clone: 129c, eBioscience™

Rat Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 17-3171-80

Code: NEW

Additional Details:
Additional Details: Weight: 0.09500kg



Disclaimers: For Research Use Only.

Product Code. 15578076

Quantity Price
1 £ 72.77 / 25µg
Estimated Shipment date
from Supplier 10-05-2017
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Description and Specification

Specification

Host Species Rat
Antigen CD317 (BST2/PDCA-1)
Isotype IgG2b
Regulatory Status RUO
Gene Alias plasmacytoid DC Ag-1, pDC Ag-1
Quantity 25μg
Concentration 0.2mg/mL
Conjugate APC
Clone eBio129c (129c)
Format Conjugated
Primary or Secondary Primary
Applications Flow Cytometry
Species Reactivity Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Monoclonal or Polyclonal Monoclonal

The eBio129c monoclonal antibody reacts with mouse PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naïve mouse which represent sa very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by the antibody 120G8.

The epitope recognized by eBio129c is distinct from eBio927; thus, the antibodies can be used to costain, purify and identify pDCs.