CD314 (NKG2D), Functional Grade, clone: CX5, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 16-5882-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The CX5 monoclonal antibody reacts with the mouse NKG2D, a lectin-like molecule expressed on both human and mouse NK cells. Mouse NKG2D binds to retinoic acid-inducible RAE-1alpha, -beta, -gamma, -delta, -ε and the minor histocompatibility molecule H60 and has the ability to costimulate multiple NK activation receptors, through the DAP12/DAP10 adaptor molecules. NKG2D is expressed by all spleen and liver NK cells, NK1.1^+ thymocytes, in vitro activated LAK cells, and a subset of splenic NKT cells.Applications Reported: The CX5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of binding of NKG2D to its ligands, RAE-1 and H60, and inhibition NKG2D-dependent NK cell-mediated cytotoxicity against NKG2D ligand-bearing tumors in vitro and in vivo .Applications Tested: The CX5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that when using this antibody for staining the secondary must be Biotin anti-rat (cat 13-4813). Other secondaries may give suboptimal staining. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
|Store at 2-8°C.|
|aqueous buffer, no sodium azide|