CD314 (NKG2D), PE, clone: 5C6, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 12-5879-41
Code : NEW
Additional Details : Weight : 0.23750kg
DescriptionDescription: The 5C6 monoclonal antibody reacts with the human NKG2D, a 42kDa lectin-like molecule expressed by NK cells, gammadelta T cells, some CD4^+ and CD8^+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gammadelta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.Applications Reported: The 5C6 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 5C6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2|
|CD314, NK cell receptor D, NKG2-D type II integral membrane protein, NKG2-D-activating NK receptor, killer cell lectin-like receptor subfamily K member 1|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
For Research Use Only.