CD314 (NKG2D), clone: 1D11, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 14-5878-82
Code : Z2
Additional Details : Weight : 0.01000kg
DescriptionDescription: The 1D11 monoclonal antibody reacts with the human NKG2D, a 42kDa lectin-like molecule expressed by NK cells, gamma delta T cells, CD8+ T cells, and some CD4+ T cells. Human NKG2D forms complexes with DAP10, a membrane adaptor protein, and has the ability to costimulate multiple NK activation receptors. The counter-receptor for human NKG2D has been identified as MICA/MICB expressed on epithelial tumors from lung, breast, kidney, ovary, prostate and colon carcinoma. 5C6 and 1D11 block binding of soluble MICA to gamma delta TCR T cell clones and inhibit lysis by these cells. 5C6 and 1D11 induced NKG2D function of redirected lysis of FcReceptor bearing P815 cells.Applications Reported: The 1D11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It has also been reported in inhibition of ligand binding in in vitro assays. Please use Functional Grade Purified 1D11 (cat. 16-5878) in functional assays. (Fluorochrome-conjugated 1D11 is recommended for use in flow cytometric analysis.).Applications Tested: The 1D11 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.09% sodium azide; pH 7.2|
|CD314, NK cell receptor D, NKG2-D type II integral membrane protein, NKG2-D-activating NK receptor, killer cell lectin-like receptor subfamily K member 1|
|Flow CytometryþFunctional AssayþImmunohistochemistry (Frozen)þImmunoprecipitation|
|Store at 2-8°C.|
For Research Use Only.