CD31 (PECAM-1), FITC, clone: 390, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-0311-81

Product Code. 15208069

Description

Description: The 390 monoclonal antibody reacts with mouse CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140kDa surface protein is expressed by endothelial cells and at low levels on all leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.Applications Reported: This 390 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: The 390 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The 390 antibody has been tested by immunohistochemistry of frozen tissue and can be used at less than or equal to 20 ug/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD31
• Classification: Monoclonal
• Concentration: 0.5mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q08481
• Gene Alias: CD31 antigen, CD31/EndoCAM, GPIIA', PECA1, PECAM-1, CD31/EndoCAM, adhesion molecule, endoCAM, platelet endothelial cell adhesion molecule
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow CytometryþImmunohistochemistry (Frozen)
• Clone: 390
• Conjugate: FITC
• Gene: Pecam1
• Format: Conjugated
• Gene Symbols: Pecam1
• Isotype: IgG2a, kappa
• Quantity: 50μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 18613

For Research Use Only.