CD309 (FLK1), Alexa Fluor 700, clone: Avas12a1, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 56-5821-81

Product Code. 15342150

Description

Description: The Avas12a1 monoclonal antibody reacts with mouse Flk-1, also known as vascular endothelial growth factor receptor 2 (VEGFR2). Flk-1 is a receptor tyrosine kinase involved in vascular endothelial tissue development and is expressed on endothelial cells during embryonic stages and some endothelial tissues in the adult.Applications Reported: This Avas12a1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This Avas12a1 antibody has been tested by flow cytometric analysis of bEND3 endothelial cell line. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.

Specifications

• Antigen: CD309 (FLK1)
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P35918
• Gene Alias: 6130401C07, Flk-1, Flk1, Krd-1, Ly73, VEGFR-2, VEGFR2, sVEGFR-2, CD309, KDR
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Murine

• Applications: Flow Cytometry
• Clone: Avas12a1
• Conjugate: Alexa Fluor 700
• Gene: Kdr
• Format: Conjugated
• Gene Symbols: Kdr
• Isotype: IgG2a, kappa
• Quantity: 50μg
• Storage Requirements: Store at 2-8°C. Do not freeze. Light-sensitive material.
• Primary or Secondary: Primary
• Gene ID (Entrez): 16542

For Research Use Only.