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CD304 (Neuropilin-1), PE-eFluor 610, clone: 3DS304M, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 61-3041-80

Code : NEW

Additional Details : Weight : 0.09500kg

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Product Code. 15500487

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance



Description: The monoclonal antibody 3DS304M recognizes mouse CD304 (Neuropilin-1), a type 1 transmembrane glycoprotein present on the surface of multiple cell types, including: regulatory T cells (Treg), NKT cells, DC, certain types of stem cells, neurons, endothelial cells, and some neoplastic cells. Neuropilin-1, in complex with plexins, serves as a co-receptor for type 3 semaphorins in growing neurons. It is also involved in the process of angiogenesis being a part of a functional receptor for VEGF in endothelial cells. In mice, Neuropilin-1 is expressed on thymus-derived natural Treg but not peripherally induced Treg. In addition, transient expression on recently activated non-regulatory T cells has been observed. Neuropilin-1 has been used as a marker of recent thymic emigrants in the mouse iNKT cell population. It has been shown that Neuropilin-1 forms a complex with TGF beta receptors, activating the latent form of TGF beta (LAP-TGF beta 1) and augmenting canonical Smad2/3 signaling.This 3DS304M antibody will recognize formaldehyde-fixed as well as methanol-treated epitopes.Applications Reported: This 3DS304M antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 3DS304M antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.


PBS with 0.09% sodium azide; pH 7.2
BDCA4, CD304, NP1, NRP, VEGF165R, neuropilin-1, transmembrane receptor, vascular endothelial cell growth factor 165 receptor
Affinity chromatography
Flow Cytometry
PE-eFluor 610
IgG2a, kappa
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.

For Research Use Only.