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CD303a, PE-Cyanine7, clone: 201A, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 25-9818-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15538676

  • £220.58 / 100 tests


Estimated Shipment date
from Supplier 30-07-2018
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Description

Description

Description: This 201A monoclonal antibody reacts with human CD303a, which is also known as CLEC4C or Blood Dendritic Cell Antigen 2 (BDCA-2). This type II transmembrane protein is expressed almost exclusively on plasmacytoid dendritic cells, which secrete type I interferon during antiviral and antitumor responses. Studies have demonstrated that CD303a binds asialo-galactosyl-oligosaccharides on invading pathogens, resulting in the activation of signaling pathways that lead to CD303a internalization and downregulation of interferon production.Applications Reported: This 201A antibody has been reported for use in flow cytometric analysis.Applications Tested: This 201A antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD303a
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
Q8WTT0
C-type lectin superfamily member 7, Blood dendritic cell antigen 2 protein, BDCA-2, Dendritic lectin, CD303 antigen, CLEC4C, BDCA2, CLECSF11, CLECSF7, DLEC, HECL, C-type lectin domain family 4 member C
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
201A
PE-Cyanine7
CLEC4C
Conjugated
CLEC4C
IgG2a, kappa
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
170482
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