CD3, eFluor 660, clone: 17A2, eBioscience™
Rat Monoclonal Antibody
Brand: Affymetrix eBioscience 50-0032-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This 17A2 antibody has also been testedy by immunohistochemistry on frozen mouse spleen and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor™ 660 is a replacement for Alexa Fluor™ 647. eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2|
|P22646, P24161, P11942, P04235|
|FLJ18683, T3E, TCRE|
|Flow CytometryþImmunocytochemistryþImmunofluorescenceþImmunohistochemistry (Frozen)|
|Cd247, Cd3d, Cd3e, Cd3g|
|IgG2b, kappa, kappa|
|4° C, store in dark, DO NOT FREEZE!|
|12500, 12501, 12502, 12503|
For Research Use Only.