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CD3, Functional Grade, clone: 17A2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 16-0032-82

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15298337

  • £75.12 / 100µg


EU Stock 5
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Description

Description

Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis and functional activity (in vitro simulation).Applications Tested: The 17A2 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2μm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.
Specifications

Specifications

CD3
Monoclonal
1mg/mL
PBS with no preservative; pH 7.2
P24161, P22646, P11942, P04235
FLJ18683, T3E, TCRE
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Functional Assay
17A2
Functional Grade
Cd3g
Purified
Cd247, Cd3d, Cd3e, Cd3g
IgG2b, kappa
100μg
Store at 2-8°C.
Primary
12500, 12501, 12502, 12503
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