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CD3, Alexa Fluor 532, clone: 17A2, eBioscience™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 58-0032-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15322160

  • £195.95 / 100µg


EU Stock 1
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Description

Description

Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser
Specifications

Specifications

CD3
Monoclonal
0.2mg/mL
PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2
P22646, P24161, P11942, P04235
FLJ18683, T3E, TCRE
Rat
Affinity chromatography
RUO
Antibody
Monoclonal
Murine
Flow Cytometry
17A2
Alexa Fluor 532
Cd3g
Conjugated
Cd247, Cd3d, Cd3e, Cd3g
IgG2b, kappa
100μg
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
12500, 12501, 12502, 12503
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