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CD3, Biotin, clone: eBioG4.18 (G4.18), eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 13-0030-82

Code : Z2

Additional Details : Weight : 0.25000kg

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Product Code. 15209399

  • £80.50 / 100Āµg


Estimated Shipment date
from Supplier 03-07-2018
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Description

Description

Description: The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. in vitro, immobilized G4.18 monoclonal antibody has been demonstrated to activate T cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. in vivo, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.Applications Reported: This eBioG4.18 (G4.18) antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.Applications Tested: This eBioG4.18 (G4.18) antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This eBioG4.18 antibody has been tested by immunohistochemistry of frozen rat tissue and can be used at less than or equal to 10 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD3
Monoclonal
0.5mg/mL
PBS with 0.09% sodium azide; pH 7.2
P19377, Q64159
FLJ18683, T3E, TCRE
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Rat
Flow CytometryĆ¾ImmunohistochemistryĆ¾Immunohistochemistry (Frozen)
eBioG4.18 (G4.18)
Biotin
Cd3g
Conjugated
Cd3d, Cd3g
IgG3, kappa
100μg
Store at 2-8°C. Do not freeze.
Primary
25710, 300678
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