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CD3, PerCP-Cyanine5.5, clone: SK7, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 45-0036-42

Code : NEW

Additional Details : Weight : 0.01000kg

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Product Code. 12672059

  • £208.21 / Each


EU Stock 15
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Disclaimers:

For Research Use Only.


Description

Description

Description: The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK7 and UCHT1 monoclonal antibodies cross-block binding, suggesting recognition of overlapping epitope. In contrast, clones OKT3 and SK7 see different epitopes. The antibody SK7 recognizes chimpanzee CD3.Applications Reported: This SK7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SK7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.03 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD3
SK7
PerCP-Cyanine5.5
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (0.03μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Leu-4, T3
IgG1
RUO
Primary
Human
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