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CD3, FITC, clone: UCHT1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-0038-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15536306

  • £49.17 / 25 tests


Estimated Shipment date
from Supplier 27-03-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: The UCHT1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This UCHT1 antibody is offered in 2 formats: -μg size: has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5μL (1μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD3
UCHT1
FITC
Conjugated
Murine
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
0.5mg/mL; 5μL (1μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Leu-4, T3
IgG1
RUO
Primary
Human
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