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CD3, FITC, clone: HIT3a, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-0039-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15556306

  • £49.17 / 25 tests


EU Stock 1
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Description

Description

Description: The HIT3a monoclonal antibody reacts with human CD3e, a 20kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR with HIT3a initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: The HIT3a antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIT3a antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD3
Monoclonal
5μl/Test
PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
P04234, P20963, P07766, P09693
FLJ18683, T3E, TCRE
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
HIT3a
FITC
CD3E
Conjugated
CD247, CD3D, CD3E, CD3G
IgG2a, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary
915, 916, 917, 919
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