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CD3, FITC, clone: HIT3a, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 11-0039-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15566306

  • £116.79 / 100 tests


EU Stock 4
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Disclaimers:

For Research Use Only.


Description

Description

Description: The HIT3a monoclonal antibody reacts with human CD3e, a 20kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR with HIT3a initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: The HIT3a antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIT3a antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (1 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD3
HIT3a
FITC
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (1μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Leu-4, T3
IgG2a
RUO
Primary
Human
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