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CD3, eFluor 450, clone: UCHT1, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 48-0038-42

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15579616

  • £156.53 / 100 tests


EU Stock 12
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Description

Description

Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: This UCHT1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This UCHT1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5μL (0.5μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is a replacement for Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD3
UCHT1
eFluor 450
Conjugated
Murine
100 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material.
Monoclonal
Flow Cytometry
5μL (0.5μg)/test
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Leu-4, T3
IgG1, kappa
RUO
Primary
Human
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