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CD3, clone: HIT3a, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 14-0039-80

Code : Z2

Additional Details : Weight : 0.01000kg

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Product Code. 15206747

  • £37.33 / 25µg


Estimated Shipment
23-02-2018
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Disclaimers:

For Research Use Only.


Description

Description

Description: The HIT3a monoclonal antibody reacts with human CD3e, a 20kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR with HIT3a initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: The HIT3a antibody has been reported for use in flow cytometric analysis.Applications Tested: The HIT3a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD3
HIT3a
Unconjugated
Purified
Murine
25μg
Store at 2-8°C.
Monoclonal
Flow Cytometry
0.5mg/mL
aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Leu-4, T3
IgG2a
RUO
Primary
Human
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