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CD3, APC-eFluor 780, clone: SK7, eBioscience™

Mouse Monoclonal Antibody

Brand:  Affymetrix eBioscience 47-0036-41

Code : NEW

Additional Details : Weight : 0.23750kg

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Product Code. 15519496

  • £86.64 / 25 tests


EU Stock 3
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Description

Description

Description: The SK7 monoclonal antibody reacts with human and chimpanzee CD3e, a 20kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. The SK7 and UCHT1 monoclonal antibodies cross-block binding, suggesting recognition of overlapping epitope. In contrast, clones OKT3 and SK7 see different epitopes. The antibody SK7 recognizes chimpanzee CD3.Applications Reported: This SK7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SK7 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor® emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
Specifications

Specifications

CD3
Monoclonal
5μl/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
P20963, P04234, P07766, P09693
FLJ18683, T3E, TCRE
Murine
Affinity chromatography
RUO
Antibody
Monoclonal
Human
Flow Cytometry
SK7
APC-eFluor 780
CD3D
Conjugated
CD247, CD3D, CD3E, CD3G
IgG1, kappa
25 Tests
Store at 2-8°C. Do not freeze. Light-sensitive material. This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling and experimental procedures.
Primary
915, 916, 917, 919
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