CD3, Alexa Fluor 700, clone: UCHT1, eBioscience™
Mouse Monoclonal Antibody
Brand: Affymetrix eBioscience 56-0038-82
Code : Z2
Additional Details : Weight : 0.25000kg
DescriptionDescription: The UCHT1 monoclonal antibody reacts with human CD3e, a 20kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: This UCHT1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This UCHT1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1μg/test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.The Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 mm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2μm post-manufacturing filtered.
|Alexa Fluor 700|
|Store at 2-8°C. Do not freeze. Light-sensitive material.|
|0.2mg/mL; 5μL (0.5μg)/test|
|aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer|
For Research Use Only.