CD294 (CRTH2) Monoclonal Antibody (BM16), eFluor™ 660, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Brand:  Affymetrix eBioscience 50-2949-42

Product Code. 15509926

Description

Description: This BM16 monoclonal antibody reacts with human CD294, which is also known as G protein-coupled receptor-44 (GPR-44) or chemoattractant receptor expressed on Th2 cells (CRTH2). CD294 is expressed on Th2 cells, some CD8+ cells, eosinophils, and basophils. Moreover, CD294 binds prostaglandin D2, which promotes leukocyte chemotaxis during allergic responses. CD294 has been reported to mediate prostaglandin D2-mediated suppression of apoptosis of Th2 cells by activating the PI3K pathway. Applications Reported: This BM16 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BM16 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD294 (CRTH2) Monoclonal antibody specifically detects CD294 (CRTH2) in Human samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD294 (CRTH2)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9Y5Y4
• Gene Symbols: PTGDR2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 11251
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: BM16
• Conjugate: eFluor 660
• Gene: PTGDR2
• Gene Alias: CD294; chemoattractant receptor homologous molecule expressed on T helper type 2 cells; chemoattractant receptor-homologous molecule expressed on TH2 cells; CRTH2; DL1R; DP2; G protein-coupled receptor 44; Gpr44; G-protein coupled receptor 44; Grp45; MGC130436; PGD2 receptor; prostaglandin D2 receptor 2; prostaglandin D2 receptor CRTH2; Ptgdr2; putative G-protein coupled receptor 44
• Host Species: Rat
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Form: Liquid

For Research Use Only.